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Please reorder the steps from A to E to illustrate the correct procedure of Colorimetry. A. The concentration of the test solution can be calculated according to the above method. Unless otherwise specified, the blank used in colorimetry refers to replace the reference solution or the test solution with the same volume of solvent, and then adding it in turn. B. The colorimetric method is used to determine the content of the test sample solution by measuring the absorbance after adding an appropriate amount of chromogenic agent. C. When colorimetric method is used for determination, several parts of gradient reference solution shall be taken and added to the same volume with solvent. After color development, the absorbance of each solution shall be determined at the wavelength under the specified monograph with corresponding reagent as blank. D. It is also advisable to determine the reference solution and the test solution at the same time. After color development, the absorbance of the reference solution and the test solution can be determined at the specified wavelength under the specified monograph with the corresponding reagent as blank. E. The standard curve shall be drawn with the absorbance as ordinate and the concentration as abscissa, and then the corresponding concentration shall be found on the standard curve according to the absorbance of the test sample, and the corresponding concentration shall be determined, then its content is determined.【试题选项缺失，请补充】

Please reorder the steps from A to E to illustrate the principles of HPLC. (A) HPLC method is to use high pressure infusion pump to pump a single solvent, mixed solvent or buffer with different polarity and different proportion as mobile phase into a stationary phase chromatographic column. (B) This method has become an important separation and analysis technology in the fields of chemistry, pharmacy, medicine, industry, agriculture, commodity inspection and forensic identification. (C) After the compounds are separated according to different principles, each component enters the detector successively, and the chromatographic signal is recorded by the recorder or integrator, to achieve the purpose of separation and analysis. (D) The chromatographic method with liquid as mobile phase is called liquid chromatography. (E) After the sample is injected through the injection valve, it is brought into the column by the mobile phase.【试题选项缺失，请补充】

To meet the ever-increasing demand for precision in the pharmaceutical field, scientists are utilizing cutting-edge technologies in pharmaceutical analysis to ensure safety and efficacy.

The ever-increasing complexity of synthetic compounds has driven innovations in spectroscopic methods for pharmaceutical analysis.

By utilizing the methodologies and techniques in physics, chemistry, biology and microbiology, pharmaceutical analysis focuses on qualitative and quantitative analysis of drugs, quality control and development of new drugs.

The broad absorption band around 3360 to 3050 cm⁻¹ is related to the stretching vibration of hydroxy of H₂O molecules.

A drug has to travel through the body in order to reach its target, so it is important to design the drug so that it is able to carry out that journey. This is an area known as pharmacokinetics.

Computer-aided drug design makes use of the structural knowledge of either the target (structure-based) or known ligands with bioactivity (ligand-based) to facilitate the determination of candidate drugs.

In terms of the Chemical Constituents, recent studies have focused more on hydrophilic compounds, and at least 50 components have been isolated and identified from the aqueous extracts of Danshen.

More than 30 diterpene compounds have been separated and identified from Danshen. Most of them are diterpene chinone compounds of the tanshinone type, including tanshinone I, IIA, IIB and other related compounds.

Pharmaceutical manufacturing enterprises should comply with GMP requirement, distribution enterprises should conform to GSP, and the government is responsible for certifying and inspecting these enterprises.

Discussing the drug registration management system, including new drug registration and registration of generic drug, imported drug and OTC products, working out and implementing the management regulations.

Nowadays, pharmacognosy is a highly specialized science, a number of the drugs used by the ancients are still employed in much the same manner by today's medical practitioners.

The purpose for which many of these medicinal substances are employed today parallels closely the use for which they were intended by our predecessors in the study of pharmacy and medicine.

An attenuated vaccine is a vaccine created by reducing the virulence of a pathogen, but still keeping it immunogenicity.

Many types of biological therapy are now available to treat a wide array of different diseases such as various forms of cancer and autoimmune diseases.